Update on Advanced Lab Services

Update on Advanced Lab Services

For those of you who are considering the 94% accurate blood culture test from Advanced Lab Services, there has been a price change.

The new prices are as follows: they are as follows: Basic Borrelia Culture $595 Monoclonal Borrelia Culture    $695. The extended 4 month culture is $150 ($845 for total recommended).

I have reported many times about Advanced Lab Services even though many have been afraid to support their services due to the unfounded rumor about contamination in their lab. Dr. Phillip Tierno, Jr. clinical professor of pathology and microbiology at NYU Langone Medical Center was hired to do an independent review of their facilities and found it to be impeccable. The following is taken from beforeitisnews.com.

The CDC never said or showed that there was in fact contamination.
They just said they could not rule it out.
To even use the word as they did in another area of the paper “probable” — those are the strongest words that they used.
They never said in fact contaminated. This is not the first time that Sanjay Gupta will have to retract his words when he looks more carefully into this.
He’s done that with marijuana. Then he saw some of the patients, and did further investigation — that’s what made him turn around. I want him to do the same thing here: come to his own conclusion by visiting the lab.
I have visited the Advanced Laboratory Services lab, so I can speak first-hand. I wrote a paper back in 1996, “Methods comparison for diagnosis of Lyme disease” (Lab Med. 1996; 27:542-546).
I was contacted by a faculty associate of mine who said, “Hey, there’s a lab in Philadelphia that could use your help in a new technique in Lyme disease.” He gave them my name and we discussed things.
I was amazed that they had a culture. Culturing is very tough. You can take some body fluids or use tissue from the erythema migrans and try to culture Borrelia, but with very low frequency. I was curious what they did that I didn’t do.
I went down there to visit and speak with the lab administration. I went through their whole facility, and if I tell you their clean rooms are clean … I also saw firsthand the spirochetes in their cultures.
They have a very intricate culture setup.
It uses collagen, a whole host of things, that even though I used a similar medium, they modified this Kelly medium, and cultured for extended periods — one month, two months, three months, four months — and did complex subcultures.
I don’t think anybody really did that to the degree that these people have done, and they have perfected it.
[The first reason to believe there is no contamination in the Advanced Laboratory Services lab:]
I have seen their cultures, and they verify the presence of Borrelia in the specimen. Of the 2000 they have done, they have a 38 percent positivity rate. By the way, if there were contamination, it would be across the board, and should be evidenced even here.
[They use something] called an immunostain. They have an antibody stain that glows under fluorescent microscopy.
Once that’s positive, they then take the material and do a PCR on it, to look at a certain segment (16S assay) of the of the genome of the bacteria, so that they can identify it as being a member of B. burgdorferi species.
Now all Borrelia, by the way — it doesn’t matter what the species is, have certain conserved signature inserts and deletions (CSIs) and conserved signature proteins (CSPs) that are indigenous to all Borrelia species, and are unique in all Borrelia species.
Some scientists have identified seven CSIs and 21 CSPs that are uniquely found in Lyme disease Borrelia.
Then there are areas where there may be some slight differences. So you look at some of these base pairs, you amplify it so you can see it better, and it’s positive or negative.
So you confirm the fact that this spirochete is indeed Borrelia. And that’s the process of identification.”



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